Functional analysis of TmHKT1;4-A2 promoter through deletion analysis provides new insight into the regulatory mechanism underlying abiotic stress adaptation

Sana Tounsi, Mohamed Najib Saïdi, Rania Abdelhedi, Kaouthar Feki, Noura Bahloul, Carine Alcon, Khaled Masmoudi, Faiçal Brini

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Main conclusion: Bioinformatic, molecular, and biochemical analysis were performed to get more insight into the regulatory mechanism by which TmHKT1;4-A2 is regulated. Abstract: HKT transporters from different plant species have been shown to play important role in plant response to salt. In previous work, TmHKT1;4-A2 gene from Triticum monococcum has been characterized as a major gene for Nax1 QTL (Tounsi et al. Plant Cell Physiol 57:2047–2057, 2016). So far, little is known about its regulatory mechanism. In this study, the promoter region of TmHKT1;4-A2 (1400 bp) was isolated and considered as the full-length promoter (PA2-1400). In silico analysis revealed the presence of important cis-acting elements related to abiotic stresses and phytohormones. Interestingly, our real-time RT-PCR analysis provided evidence that TmHKT1;4-A2 is regulated not only by salt stress but also by osmotic, heavy metal, oxidative, and hormones stresses. In transgenic Arabidopsis plants, TmHKT1;4-A2 is strongly active in vascular tissues of roots and leaves. Through 5′-end deletion analysis, we showed that PA2-1400 promoter is able to drive strong GUS activity under normal conditions and in response to different stresses compared to PA2-824 and PA2-366 promoters. These findings provide new information on the regulatory mechanism of TmHKT1;4-A2 and shed more light on its role under different stresses.

Original languageEnglish
Article number18
JournalPlanta
Volume253
Issue number1
DOIs
Publication statusPublished - Jan 2021

Keywords

  • 4-A2 promoter
  • Abiotic stress
  • Deletion analysis
  • Expression pattern
  • GUS activity
  • QTL
  • TmHKT1

ASJC Scopus subject areas

  • Genetics
  • Plant Science

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