FISH for mapping single copy genes.

Terje Raudsepp, Bhanu P. Chowdhary

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)

Abstract

During the past two decades fluorescent in-situ hybridization (FISH) has become a standard technique to directly localize, orient, and order genes in the genomes of a wide range of species. Despite the availability of a variety of probes, probe labeling and signal-detection systems, and advanced image analysis software, the core procedures used to carry out FISH remain the same. A detailed overview of these procedures, including target preparation (metaphase/interphase chromosomes and DNA fibers), probe labeling, in-situ hybridization, signal detection, and imaging, is here provided in a stepwise manner.

Original languageEnglish
Pages (from-to)31-49
Number of pages19
JournalMethods in molecular biology (Clifton, N.J.)
Volume422
DOIs
Publication statusPublished - 2008
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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