Extraction of extraradical arbuscular mycorrhizal mycelium from compartments filled with soil and glass beads

Elke Neumann, Eckhard George

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

This study presents a novel method for the extraction and quantification of extraradical mycelium (ERM) of arbuscular mycorrhizal fungi (AMF) from a substrate that simulates soil better than previously used artificial growth media. Fungal compartments were constructed from small net pots with a latticed wall and filled with a mixture of glass beads and 40 μm wet sieved soil. The net pots were surrounded by a 30-μm mesh membrane through which hyphae but not roots could grow. They were inserted into soil where a Glomus intraradices (BEG 110) colonized potato plant was growing. The ERM that had grown out from roots through the membrane was successfully collected and quantified after harvest by washing out the soil/glass bead mixture through a sieve with a mesh width of 40 μm. Concentrations of P, Zn, Cu and Mn in the AMF ERM were analysed.

Original languageEnglish
Pages (from-to)533-537
Number of pages5
JournalMycorrhiza
Volume15
Issue number7
DOIs
Publication statusPublished - Nov 2005
Externally publishedYes

Keywords

  • Extraction method
  • Extraradical mycelium
  • Fungal compartment

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Plant Science

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