Epstein Barr virus (EBV) encoded small RNAs: Targets for detection by in situ hybridisation with oligonucleotide probes

G. Khan, P. J. Coates, H. O. Kangro, G. Slavin

Research output: Contribution to journalArticlepeer-review

96 Citations (Scopus)

Abstract

Aims: To develop a rapid, sensitive, and specific non-isotopic in situ hybridisation (NISH) procedure for the detection of Epstein-Barr virus in formalin fixed, paraffin wax embedded tissues. Methods: Two low molecular weight RNAs, designated EBER-1 and EBER-2 (Epstein-Barr encoded RNA), were used: cells latently infected with EBV secrete large amounts of EBERs. The method uses digoxigenin labelled anti-sense oligonucleotides, corresponding to sequences in EBER-1 and EBER-2. Results: The use of these probes, in conjunction with high temperature microwave denaturation, ensured that the technique was considerably more sensitive than other in situ hybridisation techniques for detecting EBV. Furthermore, the hybridisation signal was morphologically distinct in that only the nucleus and not the nucleolus give a positive signal. No cross-hybridisation was observed with cells infected with other lymphotropic herpes viruses. Conclusion: The sensitivity, simplicity, and rapidity of this technique make it ideal for diagnostic use, and for studies investigating the role of this virus in neoplastic disease.

Original languageEnglish
Pages (from-to)616-620
Number of pages5
JournalJournal of Clinical Pathology
Volume45
Issue number7
DOIs
Publication statusPublished - 1992
Externally publishedYes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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