(-)-Epigallocatechin-3-gallate induces apoptosis and inhibits invasion and migration of human cervical cancer cells

Chhavi Sharma, Qurrat El Ain Nusri, Salema Begum, Elham Javed, Tahir A. Rizvi, Arif Hussain

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)

Abstract

Invasion and metastasis are the major causes of cancer-related death. Pharmacological or therapeutic interventions such as chemoprevention of the progression stages of neoplastic development could result in substantial reduction in the incidence of cancer mortality. (-)-Epigallocatechin-3-gallate (EGCG), a promising chemopreventive agent, has attracted extensive interest for cancer therapy utilizing its antioxidant, antiproliferative and inhibitory effects on angiogenesis and tumor cell invasion. In this study, we assessed the influence of EGCG on the proliferative potential of HeLa cells by cell viability assay and authenticated the results by nuclear morphological examination, DNA laddering assay and cell cycle analysis. Further we analyzed the anti-invasive properties of EGCG by wound migration assay and gene expression of MMP-9 and TIMP-1 in HeLa cells. Our results indicated that EGCG induced growth inhibition of HeLa cells in a dose- and timedependent manner. It was observed that cell death mediated by EGCG was through apoptosis. Interestingly, EGCG effectively inhibited invasion and migration of HeLa cells and modulated the expression of related genes (MMP-9 and TIMP-1) These results indicate that EGCG may effectively suppress promotion and progression stages of cervical cancer development.

Original languageEnglish
Pages (from-to)4815-4822
Number of pages8
JournalAsian Pacific Journal of Cancer Prevention
Volume13
Issue number9
DOIs
Publication statusPublished - 2012

Keywords

  • Cervical cancer
  • Epigallocatechin-3
  • Gallate
  • Invasion
  • Matrix metalloproteinases-9

ASJC Scopus subject areas

  • Epidemiology
  • Oncology
  • Public Health, Environmental and Occupational Health
  • Cancer Research

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