Enhancement of mucosal immune response against HIV-1 Gag by DNA immunization

Izumi Yoshizawa, Yoko Soda, Toshiaki Mizuochi, Sachiko Yasuda, Tahir A. Rizvi, Tsuguo Mizuochi, Toshitada Takemori, Yasuko Tsunetsugu-Yokota

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

In order to examine the feasibility of Gag-expression DNA as a potential candidate for HIV vaccine using a mouse model, we injected DNA into mice either intramuscularly or by using a gene gun. Both methods induced a low level of antibody production. However, after booster immunization with p24 protein emulsified with complete Freund's adjuvant via a footpad, we found that only the preceding intramuscular DNA immunization induced an anti-Gag Th1-type (IgG2a) antibody response, in addition to the enhancement of a Th2-type (IgG1) antibody response. Importantly, when mice were boosted intranasally with p24 and cholera toxin, intramuscular DNA injection was found to enhance both systemic and mucosal Gag-specific immune responses. These results indicate that intramuscular DNA immunization confers the inducibility of memory cells, which circulate around various mucosal tissues. Therefore, intramuscular DNA priming, followed by a mucosal booster immunization, could be considered as a regimen applicable to HIV vaccine.

Original languageEnglish
Pages (from-to)2995-3003
Number of pages9
JournalVaccine
Volume19
Issue number20-22
DOIs
Publication statusPublished - Apr 6 2001
Externally publishedYes

Keywords

  • DNA immunization
  • HIV-1 Gag
  • Mucosal immune response

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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