Electrical detection and quantification of single and mixed DNA nucleotides in suspension

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

High speed sequential identification of the building blocks of DNA, (deoxyribonucleotides or nucleotides for short) without labeling or processing in long reads of DNA is the need of the hour. This can be accomplished through exploiting their unique electrical properties. In this study, the four different types of nucleotides that constitute a DNA molecule were suspended in a buffer followed by performing several types of electrical measurements. These electrical parameters were then used to quantify the suspended DNA nucleotides. Thus, we present a purely electrical counting scheme based on the semiconductor theory that allows one to determine the number of nucleotides in a solution by measuring their capacitance-voltage dependency. The nucleotide count was observed to be similar to the multiplication of the corresponding dopant concentration and debye volume after de-embedding the buffer contribution. The presented approach allows for a fast and label-free quantification of single and mixed nucleotides in a solution.

Original languageEnglish
Article number34016
JournalScientific reports
Volume6
DOIs
Publication statusPublished - Sep 28 2016

ASJC Scopus subject areas

  • General

Fingerprint

Dive into the research topics of 'Electrical detection and quantification of single and mixed DNA nucleotides in suspension'. Together they form a unique fingerprint.

Cite this