Electrical Conduction System Remodeling in Streptozotocin-Induced Diabetes Mellitus Rat Heart

Yu Zhang, Yanwen Wang, Joseph Yanni, Mohammed Anwar Qureshi, Sunil Jit R.J. Logantha, Sarah Kassab, Mark R. Boyett, Natalie J. Gardiner, Hong Sun, Frank Christopher Howarth, Halina Dobrzynski

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Cardiovascular complications are common in type 1 diabetes mellitus (TIDM) and there is an increased risk of arrhythmias as a result of dysfunction of the cardiac conduction system (CCS). We have previously shown that, in vivo, there is a decrease in the heart rate and prolongation of the QRS complex in streptozotocin-induced type 1 diabetic rats indicating dysfunction of the CCS. The aim of this study was to investigate the function of the ex vivo CCS and key proteins that are involved in pacemaker mechanisms in TIDM. RR interval, PR interval and QRS complex duration were significantly increased in diabetic rats. The beating rate of the isolated sinoatrial node (SAN) preparation was significantly decreased in diabetic rats. The funny current density and cell capacitance were significantly decreased in diabetic nodal cells. Western blot showed that proteins involved in the function of the CCS were significantly decreased in diabetic rats, namely: HCN4, Cav1.3, Cav3.1, Cx45, and NCX1 in the SAN; RyR2 and NCX1 in the atrioventricular junction and Cx40, Cx43, Cx45, and RyR2 in the Purkinje network. We conclude that there are complex functional and cellular changes in the CCS in TIDM. The changes in the proteins involved in the function of this electrical system are expected to adversely affect action potential generation and propagation, and these changes are likely to be arrhythmogenic.

Original languageEnglish
Article number826
JournalFrontiers in Physiology
Volume10
DOIs
Publication statusPublished - Jul 8 2019

Keywords

  • arrhythmias
  • calcium handling protein
  • cardiac conduction system
  • gap junction channels
  • ion channels
  • remodeling
  • type I diabetes

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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