Effects of streptozotocin-induced type 1 diabetes mellitus on total protein concentrations and cation contents in the isolated pancreas, parotid, submandibular, and lacrimal glands of rats

Navin R. Changrani, Apurva Chonkar, Ernest Adeghate, Jaipaul Singh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

26 Citations (Scopus)

Abstract

This study investigated the effect of streptozotocin (STZ)-induced type 1 diabetes mellitus (DM) on total protein concentration and levels of sodium (Na+), potassium (K+), magnesium (Mg2+), zinc (Zn2+), copper (Cu2+), calcium (Ca2+), and iron (Fe2+) in the pancreas, parotid, submandibular, and lacrimal glands of the rat, compared to age-matched control animals. Protein concentrations were measured by the Bradford Assay, whereas levels of Na+, K +, Mg2+, Zn2+, Cu2+, Ca 2+, and Fe2+ were measured by flame photometry and atomic absorbance spectrophotometry. The results show marked changes in the characteristics of diabetic and control animals. Diabetic rats and their different glands weighed significantly (P < 0.05) less compared to age-matched controls. Diabetic rats also have significantly elevated blood glucose and significantly reduced plasma insulin, compared to controls. The results also show that the concentrations of proteins and levels of cations were significantly (P < 0.05) reduced in the pancreas, parotid, submandibular and lacrimal glands of diabetic rats, compared to glands from age-matched animals. These differences in the cation contents and protein levels in STZ-induced DM in this study, along with supporting evidences from previous studies, may provide evidence for the development of long-term complications of DM including exocrine gland deficiencies.

Original languageEnglish
Title of host publicationDiabetes Mellitus and Its Complications
Subtitle of host publicationMolecular Mechanisms, Epidemiology, and Clinical Medicine
PublisherBlackwell Publishing Inc.
Pages503-519
Number of pages17
ISBN (Print)1573316350, 9781573316354
DOIs
Publication statusPublished - Nov 2006

Publication series

NameAnnals of the New York Academy of Sciences
Volume1084
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Keywords

  • Cations
  • Diabetes
  • Lacrimal
  • Pancreas
  • Parotid
  • Rat
  • Streptozotocin
  • Submandibular
  • Total protein

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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