Effect of insulin and nimodipin on calcium signals in murine primary afferent neurons with experimental diabetes

E. P. Kostyuk, A. V. Shmigol'

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

The effect of insulin injections and long-term administration of the calcium channel blocker nimodipin on depolarization-induced calcium signals was studied in neurons isolated from the dorsal root ganglia of mice with streptozotocin-induced diabetes. Induction of diabetes in mice was followed by slowing of the calcium signal decay kinetics in small neurons (mainly related to the transmission of nociceptive signals). Subcutaneous insulin injections (1 U/kg) tended to normalize the parameters of calcium signals modified by diabetes. Preliminary 3-week-long peroral administration of nimodipin (40 mg/kg) increased the peak amplitude of depolarization-induced calcium signals in isolated neurons and caused spontaneous activity usually absent in the cells under control conditions. Kinetics of calcium transients in this case remained slow. It was concluded that hyperglycemia and related impairments of the surplus Ca2+ extrusion mechanisms play an essential role in genesis of the changes in calcium signals caused by streptozotocin-induced diabetes.

Original languageEnglish
Pages (from-to)270-275
Number of pages6
JournalNeurophysiology
Volume27
Issue number5
DOIs
Publication statusPublished - Sep 1995
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology

Fingerprint

Dive into the research topics of 'Effect of insulin and nimodipin on calcium signals in murine primary afferent neurons with experimental diabetes'. Together they form a unique fingerprint.

Cite this