Effect of endo-xylanase-containing enzyme preparations and laccase on the solubility of rye bran arabinoxylan

Roger Andersson, Christina Eliasson, Mangala Selenare, Afaf Kamal-Eldin, Per Åman

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Three commercial enzyme preparations with endo-xylanase activity, namely Bio-Feed Wheat, Bio-Feed Plus and Grindamyl H 640, and laccase have been tested for their effects on the solubilisation of arabinoxylan (AX) in rye bran or autoclaved rye bran. Autoclaving efficiently increased the availability of AX for enzymatic degradation. Both Bio-Feed Wheat (a monocomponent enzyme) and Bio-Feed Plus (a multicomponent preparation with different enzymatic activities) efficiently degraded the autoclaved rye bran AX into lower-molecular-weight fragments. As much as 70% of the xylose residues and 58% of the arabinose residues in the autoclaved bran were soluble after treatment with Bio-Feed Plus; the weight - average molecular weight of the detectable portion of these soluble polymers was 104000Da. Grindamyl H 640 solubilised only a small fraction of the AX in autoclaved rye bran; the molecular weight of these soluble fragments was higher than that of those released by the Bio-Feed xylanases. Addition of laccase during treatment with Bio-Feed Wheat or Grindamyl H 640 decreased the yield of water-soluble AX.

Original languageEnglish
Pages (from-to)617-623
Number of pages7
JournalJournal of the Science of Food and Agriculture
Volume83
Issue number7
DOIs
Publication statusPublished - May 15 2003
Externally publishedYes

Keywords

  • Arabinoxylan
  • Endo-xylanase
  • Laccase
  • Rye bran
  • Solubility
  • Structure

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Agronomy and Crop Science
  • Nutrition and Dietetics

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