Dynamic proteomic profile of potato tuber during its in vitro development

Jae Woong Yu, Jong Soon Choi, Chandrama Prakash Upadhyaya, Sang Oh Kwon, Mayank Anand Gururani, Akula Nookaraju, Ju Hyun Nam, Chi Won Choi, Seung Il Kim, Hemavathi Ajappala, Hyun soon Kim, Jae Heung Jeon, Se Won Park

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Potato tuberization is a complicated biochemical process, which is dependent on external environmental factors. Tuber development in potato consists of a series of biochemical and morphological processes at the stolon tip. Signal transduction proteins are involved in the source-sink transition during potato tuberization. In the present study, we examined protein profiles under in vitro tuber-inducing conditions using a shotgun proteomic approach involving denaturing gel electrophoresis and liquid chromatography-mass spectrometry. A total of 251 proteins were identified and classified into 9 groups according to distinctive expression patterns during the tuberization stage. Stolon stage-specific proteins were primarily involved in the photosynthetic machinery. Proteins specific to the initial tuber stage included patatin. Proteins specific to the developing tuber stage included 6-fructokinase, phytoalexin-deficient 4-1, metallothionein II-like protein, and malate dehydrogenase. Novel stage-specific proteins identified during in vitro tuberization were ferredoxin-NADP reductase, 34. kDa porin, aquaporin, calmodulin, ripening-regulated protein, and starch synthase. Superoxide dismutase, dehydroascorbate reductase, and catalase I were most abundantly expressed in the stolon; however, the enzyme activities of these proteins were most activated at the initial tuber. The present shotgun proteomic study provides insights into the proteins that show altered expression during in vitro potato tuberization.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalPlant Science
Volume195
DOIs
Publication statusPublished - Oct 2012
Externally publishedYes

Keywords

  • Potato tuber
  • Shotgun proteomics
  • Stage-specific protein
  • Tuberization

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

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