Distribution and heterogeneity of immunoreactive cholecystokinin (CCK) in the mucosa of the porcine gastrointestinal tract

A. J. Bacarese-Hamilton, T. E. Adrian, S. R. Bloom

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

The concentration and molecular nature of cholecystokinin-like immunoreactivity (CCK-LI) in extracts of porcine intestinal mucosa were determined using sequence-specific radioimmunoassays. Highest CCK concentrations were measured in duodenal mucosa (258 ± 60 pmol/g in the distal duodenum) followed by jejunal mucosa (204 ± 36 pmol/g in the proximal jejunum) and pylorus (51 ± 9 pmol/g). All other gastrointestinal regions proximal to the pylorus and distal to the jejunum contained less than 20 pmol/g. Pancreas contained less than 1 pmol/g. Gel chromatography in 6 M urea revealed four immunoreactive forms and this was confirmed by reverse-phase high-pressure liquid chromatography (HPLC). The predominant molecular form in acid extracts of duodenal mucosa resembled CCK-33 although high concentrations of the larger CCK form ('CCK-58') and of the form intermediate in size between CCK-33 and CCK-8 were measured. A molecular form resembling CCK-8 was the principal form in neutral extracts of the duodenum.

Original languageEnglish
Pages (from-to)289-298
Number of pages10
JournalRegulatory Peptides
Volume9
Issue number4
DOIs
Publication statusPublished - Nov 1984
Externally publishedYes

Keywords

  • cholecystokinin
  • distribution
  • gel chromatography
  • heterogeneity
  • reverse-phase high-pressure liquid chromatography
  • specific radioimmunoassay

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience

Fingerprint

Dive into the research topics of 'Distribution and heterogeneity of immunoreactive cholecystokinin (CCK) in the mucosa of the porcine gastrointestinal tract'. Together they form a unique fingerprint.

Cite this