Differential degradation and detoxification of an aromatic pollutant by two different peroxidases

Aysha Hamad Alneyadi, Iltaf Shah, Synan F. Abuqamar, Syed Salman Ashraf

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Enzymatic degradation of organic pollutants is a new and promising remediation approach. Peroxidases are one of the most commonly used classes of enzymes to degrade organic pollutants. However, it is generally assumed that all peroxidases behave similarly and produce similar degradation products. In this study, we conducted detailed studies of the degradation of a model aromatic pollutant, Sulforhodamine B dye (SRB dye), using two peroxidases—soybean peroxidase (SBP) and chloroperoxidase (CPO). Our results show that these two related enzymes had different optimum conditions (pH, temperature, H2O2 concentration, etc.) for efficiently degrading SRB dye. High-performance liquid chromatography and liquid chromatography –mass spectrometry analyses confirmed that both SBP and CPO transformed the SRB dye into low molecular weight intermediates. While most of the intermediates produced by the two enzymes were the same, the CPO treatment produced at least one different intermediate. Furthermore, toxicological evaluation using lettuce (Lactuca sativa) seeds demonstrated that the SBP-based treatment was able to eliminate the phytotoxicity of SRB dye, but the CPO-based treatment did not. Our results show, for the first time, that while both of these related enzymes can be used to efficiently degrade organic pollutants, they have different optimum reaction conditions and may not be equally efficient in detoxification of organic pollutants.

Original languageEnglish
Article number31
JournalBiomolecules
Volume7
Issue number1
DOIs
Publication statusPublished - Mar 18 2017

Keywords

  • Chloroperoxidase
  • Enzymatic remediation
  • Organic pollutants
  • Soybean peroxidase
  • Sulforhodamine B

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint

Dive into the research topics of 'Differential degradation and detoxification of an aromatic pollutant by two different peroxidases'. Together they form a unique fingerprint.

Cite this