Different properties of caffeine-sensitive Ca2+ stores in peripheral and central mammalian neurones

Anatoly Shmigol, Sergej Kirischuk, Platon Kostyuk, Alexej Verkhratsky

Research output: Contribution to journalArticlepeer-review

74 Citations (Scopus)

Abstract

Using indo-1 based microfluorometry for measuring the cytoplasmic free calcium concentration ([Ca2+]i), the properties of caffeine-induced Ca2+ release from internal stores were studied in rat cultured central and peripheral neurones, including dorsal root ganglia (DRG) neurones, neurones from nucleus cuneatus, CA1 and CA3 hippocampal region and pyramidal neocortical neurones. Under resting conditions the Ca2+ content of internal stores in DRG neurones was high enough to produce caffeine-triggered [Ca2+]i transients. Caffeine-induced Ca2+ release depleted internal stores in DRG neurones, but they refilled themselves spontaneously up to 81.4±5.67 % within 10 minutes. In contrast, in all types of central neurones the resting Ca2+ content of internal stores was low, but the stores could be charged by transmembrane Ca2+ influx through voltage-operated calcium channels. After charging, the stores in central neurones spontaneously lost releasable calcium content and within 10 minutes they emptied again. We suggest that in sensory neurones calcium stores are continuously filled by releasable calcium and after discharge they can refill themselves spontaneously, while in central neurones internal calcium stores can be charged by releasable calcium only transiently.

Original languageEnglish
Pages (from-to)174-176
Number of pages3
JournalPflügers Archiv European Journal of Physiology
Volume426
Issue number1-2
DOIs
Publication statusPublished - Jan 1994
Externally publishedYes

Keywords

  • caffeine
  • calcium stores
  • cytoplasmic calcium
  • neurones

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

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