Determination of vancomycin content in dried blood spots for therapeutic drug monitoring

Mutasim Al-Ghazawi, Nour El Huda Daoud, Kamal A. Hadidi, Muhammed Alzweiri, Salahdein Aburuz

Research output: Contribution to journalArticlepeer-review

Abstract

Blood spot collection is a simple method to obtain specimens for therapeutic monitoring of drugs, assessing drug adherence, and preventing any potential drug toxicity. A simple chromatographic method for the determination of vancomycin in dried blood spots (DBS) has been developed and validated. Intra and inter-day precisions (measured by CV%) were less than 11.5% and the accuracy deviation was less than 11%. The limits of detection (LOD) and quantification (LOQ) were 1 and 10 ng/mL, respectively. Interestingly, LOQ is smaller than the smallest vancomycin monitoring level. Stability analysis shows that vancomycin is stable for at least three weeks when stored at 4 to 8°C. The high sensitivity of detection and short time of analysis (less than 8 min), combining with the simplicity of the procedure made the method applicable for monitoring vancomycin in dried blood spots, which might be implemented in routine clinical settings.

Original languageEnglish
Pages (from-to)3-10
Number of pages8
JournalActa Poloniae Pharmaceutica - Drug Research
Volume78
Issue number1
DOIs
Publication statusPublished - 2021

Keywords

  • Blood spot
  • Chromatography
  • Determination of vancomycin
  • Therapeutic drug monitoring

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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