Determination of androstenone levels in porcine plasma by LC-MS/MS

G. Chen, L. Ren, S. M. Yang, J. Qiu, A. Kamal-Eldin, K. Lundström

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Androstenone (5α-androst-16-en-3-one) is a major component in boar taint. Here, a liquid chromatography-tandem mass spectrometry method was developed to analyse androstenone in porcine plasma to facilitate studies of its transport from pig testes to adipose tissues. The method used androstanone (5α-andro-stan-3-one) as internal standard, Oasis® MCX solid-phase extraction, C18 reversed-phase column, and API 5000 Triple Quadrupole mass spectrometry with positive electrospray ionisation interface operating in the multiple reaction monitoring mode. Incubation of the plasma samples with β-glucuronidase/arylsulfatase revealed an increase in androstenone yield indicating the presence of a conjugated form of its 3-enol isomer. With this method the limit of detection (LOD) was 1.0 ng and the limit of quantification (LOQ) was 3.3 ng per mL of plasma. In conclusion, the presented method is sensitive and reproducible and thus suitable for accurate quantification of androstenone levels in a small volume of porcine plasma.

Original languageEnglish
Pages (from-to)1278-1282
Number of pages5
JournalFood Chemistry
Volume122
Issue number4
DOIs
Publication statusPublished - Oct 15 2010
Externally publishedYes

Keywords

  • Androstenone
  • LC-MS/MS
  • Porcine plasma
  • Solid-phase extraction

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

Fingerprint

Dive into the research topics of 'Determination of androstenone levels in porcine plasma by LC-MS/MS'. Together they form a unique fingerprint.

Cite this