Construction of chromosome-specific paints for meta- and submetacentric autosomes and the sex chromosomes in the horse and their use to detect homologous chromosomal segments in the donkey

Terje Raudsepp, Bhanu P. Chowdhary

Research output: Contribution to journalArticlepeer-review

51 Citations (Scopus)

Abstract

A pilot study comparing horse and donkey karyotypes on a molecular basis was initiated using the chromosomal microdissection approach. All equine meta- and submetacentric chromosomes, viz. ECA1 to ECA13 and the X and Y chromosomes, were microdissected. The DNA was PCR amplified, non-radioactively labelled and used as probes on equine metaphase chromosomes to confirm their origin. Once tested, the paints were used as probes on donkey metaphase chromosomes to detect homologous chromosomal segments between the two species. The results not only detected conservation of whole chromosome and/or arm synteny between the two karyotypes, but also highlighted varying degrees of rearrangements. The findings also enable deduction of homology between parts of donkey and human karyotypes. In light of the molecular evidence, this study examines the accuracy of the available comparative cytogenetic data between horse and donkey.

Original languageEnglish
Pages (from-to)103-114
Number of pages12
JournalChromosome Research
Volume7
Issue number2
DOIs
Publication statusPublished - 1999
Externally publishedYes

Keywords

  • Chromosome microdissection
  • Donkey
  • Horse
  • Human
  • Zoo-FISH

ASJC Scopus subject areas

  • Genetics

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