Combined estrogen and ghrelin administration decreases expression of p27kip1 and proportion of isomyosin type I in the striated urethral and anal sphincters and levator ani of old ovariectomized rats

Diaa E.E. Rizk, Hazem A. Hassan, Ahmed H. Al-Marzouqi, Mohammed Shafiullah, Mohamed A. Fahim

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

We compared estrogen and/or ghrelin effects on pelvic floor muscles in old versus young adult ovariectomized rats. Ovariectomized Fisher 344 rats (18 and 3 months old, n= 24 × 2) received 42 daily intraperitoneal 17-β estradiol (10 μg kg-1), ghrelin (2 μg kg-1), both, or vehicle (n = 6 × 4/group). Cytoplasmic p27kip1 expression and isomyosin I proportion in striated urethral and anal sphincters and levator ani were measured, respectively, by Western blot analysis and gel electrophoresis with immunohistochemistry of muscle ghrelin receptors and radioimmunoassay of circulating growth hormone. In young adult rats, estrogen significantly decreased cytoplasmic p27kip1 and isomyosin I signal intensities. In old rats, ghrelin and estrogen/ghrelin significantly decreased both intensities with greater estrogen/ghrelin effect. Ghrelin receptors were not immunostained in any muscle. Estrogen and/or ghrelin significantly increased or decreased, respectively, circulating growth hormone in old and young adult rats. Estrogen/ghrelin administration reversed pelvic floor muscle ageing changes in old ovariectomized rats through growth hormone production.

Original languageEnglish
Pages (from-to)1363-1369
Number of pages7
JournalInternational Urogynecology Journal
Volume19
Issue number10
DOIs
Publication statusPublished - 2008

Keywords

  • Ageing
  • Ghrelin
  • Isomyosin
  • Menopause
  • Rat
  • p27

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Urology

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