Combination of 2DE and LC for plant proteomics analysis

Sami Irar, Faiçal Brini, Khaled Masmoudi, Montserrat Pagès

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

The use of analytical biochemical techniques with different separation properties allows us to better understand the proteome. To demonstrate this we have used two different methodologies to analyze embryos from a Tunisian cultivar of durum wheat (Triticum durum Desf.), variety Oum Rabiaa. We compared conventional 2D electrophoresis with liquid-phase chromatography. Our results show that a similar number of proteins were detected with both techniques. However, analysis of protein resolution at different pH ranges showed significant differences. By using a large pH gradient we observed that liquid chromatography presents higher resolution at extreme pH, either acidic or basic. Conversely, 2DE is more resolutive at intermediate pH (pH 5-6.5). Taking these results in consideration, we propose that 2DE and liquid chromatography are complementary methods to analyze complex protein extracts and can be used in parallel to acquire a wider perspective and a better understanding of the embryo proteome.

Original languageEnglish
Title of host publicationPlant Proteomics
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages131-140
Number of pages10
ISBN (Print)9781627036306
DOIs
Publication statusPublished - 2014
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1072
ISSN (Print)1064-3745

Keywords

  • 2D electrophoresis
  • Comparative plant proteomics
  • Liquid chromatography
  • ProteomeLab PF-2D
  • Wheat embryo proteome

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'Combination of 2DE and LC for plant proteomics analysis'. Together they form a unique fingerprint.

Cite this