Characterization of the plant Notchless homolog, a WD repeat protein involved in seed development

Sier Ching Chantha, B. Starling Emerald, Daniel P. Matton

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

We have isolated a plant NOTCHLESS (NLE) homolog from the wild potato species Solanum chacoense Bitt., encoding a WD-repeat containing protein initially characterized as a negative regulator of the Notch receptor in animals. Although no Notch signaling pathway exists in plants, the NLE gene is conserved in animals, plants, and yeast. Overexpression of the plant ScNLE gene in Drosophila similarly affected bristle formation when compared to the overexpression of the endogenous Drosophila NLE gene, suggesting functional conservation. Expression analyses showed that the ScNLE gene was fertilization-induced and primarily expressed in ovules after fertilization, mainly in the integumentary tapetum (endothelium). Significant expression was also detected in the shoot apex. Promoter deletion analysis revealed that the ScNLE promoter had a complex modulatory architecture with both positive, negative, and tissue specific regulatory elements. Transgenic plants with reduced levels of ScNLE transcripts displayed pleitotropic phenotypes including a severe reduction in seed set, consistent with ScNLE gene expression pattern.

Original languageEnglish
Pages (from-to)897-912
Number of pages16
JournalPlant Molecular Biology
Volume62
Issue number6
DOIs
Publication statusPublished - Dec 2006
Externally publishedYes

Keywords

  • Fertilization
  • Notchless
  • Ovule
  • Seed
  • WD-repeat protein

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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