Characterization of bradykinin-related peptides generated in the plasma of six sarcopterygian species (African Lungfish, Amphiuma, Coachwhip, Bullsnake, Gila Monster, and Gray's Monitor)

Zhihong Li, Stephen M. Secor, Valentine A. Lance, Maria A. Masini, Mauro Vallarino, J. Michael Conlon

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Incubation of heat-denatured plasma from six species occupying different evolutionary positions within the Sarcopterygian lineage [the dipnoan, Protopterus annectens (African lungfish); the urodele, Amphiuma tridactylum (three-toed amphiuma); the colubrid snakes, Pituophis melanoleucus sayi (bullsnake) and Masticophis flagellum (coachwhip); and the lizards Heloderma suspectum (Gila monster) and Varanus Grayi (Gray's monitor)] with trypsin generated bradykinin-related peptides that were detected by radioimmunoassay using an antiserum raised against mammalian bradykinin (BK). The peptides were purified by HPLC and their primary structures were established as lungfish [Tyr1,Gly2,Ala7,Pro8]BK, amphiurea [Phe1,Ile2,Leu5]BK, bullsnake and coachwhip [Val1,Thr6]BK, Gila monster [Leu2,Thr6]BK, and Grays monitor [Thr6]BK. Monitor BK is identical to the peptide generated in turtle and alligator plasma and coachwhip/bullsnake BK shows one amino acid substitution (Ala1 → Val) compared with the peptide generated in the plasma of the python. The data provide further evidence for the widespread occurrence of a kallikrein-kininogen system in nonmammalian vertebrates but indicate that the primary structure of BK has been poorly conserved during evolution.

Original languageEnglish
Pages (from-to)108-114
Number of pages7
JournalGeneral and Comparative Endocrinology
Volume112
Issue number1
DOIs
Publication statusPublished - Oct 1998
Externally publishedYes

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Endocrinology

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