The primary structure of pancreatic polypeptide from the teleostean fish, Cottus scorpius (daddy sculpin) was established as: Y P P Q P E S P G G N A S P E D W A K Y H A A V R H Y V N L I T R Q R Y NH2. The presence of a COOH-terminally α-amidated amino acid was established using an HPLC method of general applicability. Although the peptide shows strong homology towards anglerfish pancreatic polypeptide (86%), homology towards porcine peptide YY (PYY) (61%) and porcine neuropeptide Y (NPY) (61%) was greater than towards porcine pancreatic polypeptide (PP) (47%). This result supports suggestions that the gene duplication events which led to PP, NPY and PYY formation took place after the time of divergence of fish and mammals.
- primary structure
- teleost pancreatic islet
ASJC Scopus subject areas
- Clinical Biochemistry
- Cellular and Molecular Neuroscience