Antioxidant potentials and ajmalicine accumulation in Catharanthus roseus after treatment with giberellic acid

C. Abdul Jaleel, R. Gopi, P. Manivannan, B. Sankar, A. Kishorekumar, R. Panneerselvam

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)

Abstract

Changes in antioxidant potentials and indole alkaloid, ajmalicine, production were studied in Catharanthus roseus (L.) G. Don. plants under treatment with gibberellic acid (GA3). The GA3 treatments were given in two ways, foliar spray and soil drenching methods on 30, 45, 60 and 75 days after planting (DAP). The plants were uprooted randomly on 90 DAP and separated into root, stem and leaves and used for analyses. The antioxidant potential was studied in terms of non-enzymatc antioxidant molecules like ascorbic acid (AA), α-tocopherol (α-toc) and reduced glutathione (GSH) and activities of antioxidant enzyme, viz., superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT). The alkaloid ajmalicine was extracted and estimated from roots of both control and treated plants. It was found that, GA3 has a profound effect upon the antioxidant potentials and it caused a significant enhancement in the production of ajmalicine when compared to untreated control as well as foliar-sprayed plants. There was no significant enhancement in GSH and ajmalicine content under GA3 foliar spray in C. roseus. These preliminary results suggest that, the application of GA3 may be a useful tool to increase the antioxidant potential and alkaloid production in medicinal plants like C. roseus.

Original languageEnglish
Pages (from-to)195-200
Number of pages6
JournalColloids and Surfaces B: Biointerfaces
Volume60
Issue number2
DOIs
Publication statusPublished - Nov 15 2007
Externally publishedYes

Keywords

  • Ajmalicine
  • Ascorbate peroxidase
  • Ascorbic acid
  • Catalase
  • Catharanthus roseus
  • Gibberellic acid
  • Reduced glutathione
  • Superoxide dismutase
  • α-Tocopherol

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Fingerprint

Dive into the research topics of 'Antioxidant potentials and ajmalicine accumulation in Catharanthus roseus after treatment with giberellic acid'. Together they form a unique fingerprint.

Cite this