Analysis of SARS-CoV-2 RNA-dependent RNA polymerase as a potential therapeutic drug target using a computational approach

Syed Ovais Aftab, Muhammad Zubair Ghouri, Muhammad Umer Masood, Zeshan Haider, Zulqurnain Khan, Aftab Ahmad, Nayla Munawar

Research output: Contribution to journalArticlepeer-review

88 Citations (Scopus)

Abstract

Background: The Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) outbreak originating in Wuhan, China, has raised global health concerns and the pandemic has now been reported on all inhabited continents. Hitherto, no antiviral drug is available to combat this viral outbreak. Methods: Keeping in mind the urgency of the situation, the current study was designed to devise new strategies for drug discovery and/or repositioning against SARS-CoV-2. In the current study, RNA-dependent RNA polymerase (RdRp), which regulates viral replication, is proposed as a potential therapeutic target to inhibit viral infection. Results: Evolutionary studies of whole-genome sequences of SARS-CoV-2 represent high similarity (> 90%) with other SARS viruses. Targeting the RdRp active sites, ASP760 and ASP761, by antiviral drugs could be a potential therapeutic option for inhibition of coronavirus RdRp, and thus viral replication. Target-based virtual screening and molecular docking results show that the antiviral Galidesivir and its structurally similar compounds have shown promise against SARS-CoV-2. Conclusions: The anti-polymerase drugs predicted here - CID123624208 and CID11687749 - may be considered for in vitro and in vivo clinical trials.

Original languageEnglish
Article number275
JournalJournal of Translational Medicine
Volume18
Issue number1
DOIs
Publication statusPublished - Jul 7 2020

Keywords

  • Active site
  • Homology modeling
  • Molecular Docking
  • Phylogenetic tree
  • RdRp
  • SARS-CoV-2

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint

Dive into the research topics of 'Analysis of SARS-CoV-2 RNA-dependent RNA polymerase as a potential therapeutic drug target using a computational approach'. Together they form a unique fingerprint.

Cite this