An infection-relevant transcriptomic compendium for salmonella enterica serovar typhimurium

Carsten Kröger, Aoife Colgan, Shabarinath Srikumar, Kristian Händler, Sathesh K. Sivasankaran, Disa L. Hammarlöf, Rocío Canals, Joe E. Grissom, Tyrrell Conway, Karsten Hokamp, Jay C.D. Hinton

Research output: Contribution to journalArticlepeer-review

282 Citations (Scopus)

Abstract

Bacterial transcriptional networks consist of hundreds of transcription factors and thousands of promoters. However, the true complexity of transcription in a bacterial pathogen and the effect of the environments encountered during infection remain to be established. We present a simplified approach for global promoter identification in bacteria using RNA-seq-based transcriptomic analyses of 22 distinct infection-relevant environmental conditions. Individual RNA samples were combined to identify most of the 3,838 Salmonella enterica serovar Typhimurium promoters in just two RNA-seq runs. Individual in vitro conditions stimulated characteristic transcriptional signatures, and the suite of 22 conditions induced transcription of 86% of all S. Typhimurium genes. We highlight the environmental conditions that induce the Salmonella pathogenicity islands and present a small RNA expression landscape of 280 sRNAs. This publicly available compendium of environmentally controlled expression of every transcriptional feature of S. Typhimurium constitutes a useful resource for the bacterial research community.

Original languageEnglish
Pages (from-to)683-695
Number of pages13
JournalCell Host and Microbe
Volume14
Issue number6
DOIs
Publication statusPublished - Dec 11 2013
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology

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