Amperometric monitoring of lactate accumulation in rabbit ischemic myocardium

Sayed A.M. Marzouk, Vasile V. Cosofret, Richard P. Buck, Hua Yang, Wayne E. Cascio, Saad S.M. Hassanc

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

Fabrication and characterization of miniature, flexible, planar biosensors for monitoring L-lactate accumulation in an ischemic myocardium are described. Three configurations of Au-based electrodes were fabricated by a photolithographic technique on flexible polyimide Kapton® foil. All sensors are based on an immobilized lactate oxidase with amperometric detection of the enzymatically produced hydrogen peroxide at a platinum-electroplated-gold base electrode polarized at 0.5 V versus Ag/AgCl. An inner electropolymeric layer is used to prevent electrode fouling and to reject the interference effects of easily oxidizable molecules. In addition, a diffusion controlling outer layer that greatly enhances the linear dynamic range of the sensor, is obtained by casting a polyurethane external film. The developed sensor was evaluated in vitro and proved to have high selectivity, good operational stability, good accuracy and precision (average recovery = 102.3 ± 0.4% for control sera), fast response time (t95 = 20 s) and high upper limit of the linear dynamic range (25-80 mM, with sensitivity of 1.7-0.4 nA mM-1, respectively at PO2 = 15 mmHg). Subsequently, the sensor was brought into direct contact with the surface of the rabbit papillary muscle and used for continuous quantitative monitoring of extracellular lactate accumulation during no-flow ischemia.

Original languageEnglish
Pages (from-to)1527-1541
Number of pages15
JournalTalanta
Volume44
Issue number9
DOIs
Publication statusPublished - Sep 1997
Externally publishedYes

Keywords

  • Amperometric biosensors
  • Ischemia
  • Lactate monitoring
  • Papillary muscle

ASJC Scopus subject areas

  • Analytical Chemistry

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