Amaranth proteins as potential source of bioactive peptides with enhanced inhibition of enzymatic markers linked with hypertension and diabetes

Hina Kamal, Priti Mudgil, Bincy Bhaskar, Ajayi Feyisola Fisayo, Chee Yuen Gan, Sajid Maqsood

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The health and environmental concerns are provoking changes in human diets and plant-based proteins are being considered as sustainable source of proteins compared to animal-derived proteins. In line with this, the current study investigated amaranth protein isolate (API) and hydrolysates (APHs) for in-vitro angiotensin-I converting enzyme (ACE), dipeptidyl peptidase-IV (DPP-IV), and α-glucosidase (AG) inhibitory activities. Amaranth protein hydrolysates (APHs) were generated by bromelain, chymotrypsin, and pronase E for 2, 4, and 6 h. All APHs, especially bromelain-4 h hydrolysate (B4) displayed enhanced ACE, DPP-IV, and AG inhibition compared to API and other APHs. About 116 peptides were identified in B4 by LC-MS-QToF and 17 peptides were predicted to be bioactive. Six of these peptides were predicted to possess high ACE inhibiting potential, while peptides FPFPPTLGY and FPFPR were found to bind to the highest number of active hotspots of DPP-IV and AG, respectively. Overall, this study demonstrated that APHs could be a potential source of antidiabetic and antihypertensive peptides for functional food formulation.

Original languageEnglish
Article number103308
JournalJournal of Cereal Science
Volume101
DOIs
Publication statusPublished - Sep 2021

Keywords

  • ACE
  • Amaranth
  • Antidiabetic peptides
  • Antihypertensive
  • DPP-IV
  • Protein hydrolysates
  • α-glucosidase

ASJC Scopus subject areas

  • Food Science
  • Biochemistry

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