Affinity screening by packed capillary high performance liquid chromatography using molecular imprinted sorbents: II. Covalent imprinted polymers

Mohammad A. Khasawneh, Patrick T. Vallano, Vincent T. Remcho

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)

Abstract

This study concentrates on the production of covalent molecular imprint polymers (MIPs) as highly selective sorbents for nortriptyline (NOR), a representative tricyclic antidepressant (TCA). The functionalized template contains a polymerizable 4-vinylphenyl carbamate moiety used to bind the template molecule to the polymer matrix. Polymerization with a cross-linker followed by hydrolytic cleavage of the labile carbamate functionality leaves an MIP with selective binding sites capable of binding template through hydrogen bonding interactions. Demonstrated chromatographically through a "selection index", these MIPs showed high selectivity for the template molecule (NOR) among a library of structurally similar compounds. The recognition was found to correlate with structural similarity to the template compound. A direct comparison between covalent and non-covalent molecular imprinting strategies reveals a great deal of improvement in the peak shape of the retained compound resulting from covalent imprinting (evidenced by peak asymmetry factors As).

Original languageEnglish
Pages (from-to)87-97
Number of pages11
JournalJournal of Chromatography A
Volume922
Issue number1-2
DOIs
Publication statusPublished - Jul 13 2001
Externally publishedYes

Keywords

  • Covalent imprinting
  • Molecular imprinted sorbents
  • Molecular imprinting
  • Nortriptyline
  • Peak shape

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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