Adsorption of lipase on hollow fiber membrane chips

Marwan M. Shamel, Rohailistura B. Azaha, Sulaiman Al-Zuhair

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The amount of lipase from Mucor miehei adsorption on ultrafiltration polysulfone hollow fiber membrane chips has been determined using different lipase concentrations at three different temperatures, namely 30, 35, and 40°C. It was experimentally shown that adsorption of lipase increases with temperature. The results were used to evaluate the constants found in the Langmuir adsorption isotherm model coupled with the Van't Hoff's relationship. A temperature dependence correlation for the amount of adsorbed lipase activity, alip,ads, and that present in the supernatant solution, a lip,free was determined. The effect of varying the concentration on a cross-linking agent, namely, glutaraldehyde, to the membrane chips was also tested. It was found that, under the same operating conditions, the amount of lipase adsorbed on polysulfone membranes was increased dramatically after pre-treating the membrane with 1% Glutaraldehyde. However, increasing the concentration of the cross-linking agent has a low effect on the amount of lipase adsorbed.

Original languageEnglish
Pages (from-to)423-433
Number of pages11
JournalArtificial Cells, Blood Substitutes, and Immobilization Biotechnology
Volume33
Issue number4
DOIs
Publication statusPublished - 2005
Externally publishedYes

Keywords

  • Adsorption
  • Glutaraldehyde
  • Langmuir adsorption isotherm
  • Lipase
  • Membrane reactors
  • Polysulphone

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering

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