Activation domain-mediated targeting of the SWI/SNF complex to promoters stimulates transcription from nucleosome arrays

Kristen E. Neely, Ahmed H. Hassan, Annika E. Wallberg, David J. Steger, Bradley R. Cairns, Anthony P.H. Wright, Jerry L. Workman

Research output: Contribution to journalArticlepeer-review

210 Citations (Scopus)

Abstract

The yeast SWI/SNF complex is required for the transcription of several yeast genes and has been shown to alter nucleosome structure in an ATP-dependent reaction. In this study, we show that the complex stimulated in vitro transcription from nucleosome templates in an activation domain-dependent manner. Transcription stimulation by SWI/SNF required an activation domain with which it directly interacts. The acidic activation domains of VP16, Gcn4, Swi5, and Hap4 interacted directly with the purified SWI/SNF complex and with the SWI/SNF complex in whole-cell extracts. The similarity of activation domain interactions and transcriptional stimulation between SWI/SNF and the SAGA histone acetyltransferase complex may account for their apparent overlapping functions in vivo.

Original languageEnglish
Pages (from-to)649-655
Number of pages7
JournalMolecular Cell
Volume4
Issue number4
DOIs
Publication statusPublished - Oct 1999
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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