A muscarinic receptor type in human lymphocytes: A comparison of 3H-QNB binding to intact lymphocytes and lysed lymphocyte membranes

A. Adem, A. Nordberg, P. Slanina

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

Human blood lymphocytes from normal blood donors exhibited specific binding of the muscarinic antagonist 3H-quinuclidinyl benzilate (3H-QNB). The 3H-QNB binding to intact viable lymphocytes as well as to lysed lymphocyte membranes "P2" was saturable and displaceable by both muscarinic agonists and antagonists. For the lysed lymphocyte membranes "P2" a single binding site with a Bmax of 109 pmol/g protein and a Kd of 15 nM was obtained. Intact viable lymphocytes also showed one binding site with a Kd of 24 nM and a Bmax of 1556 pmol/g protein. The higher Bmax value might be explained in terms of uptake of the ligand when using intact cells or through loss of binding sites when using lysed lymphocyte membranes "P2". IC50 values were lower by a factor of 102 for atropine and scopolamine and by 104 for pirenzepine when lysed lymphocyte membranes "P2" were used instead of intact viable lymphocytes.

Original languageEnglish
Pages (from-to)1359-1368
Number of pages10
JournalLife Sciences
Volume38
Issue number15
DOIs
Publication statusPublished - Apr 14 1986
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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