A human-horse comparative map based on equine BAC end sequences

Tosso Leeb, Claus Vogl, Baoli Zhu, Pieter J. de Jong, Matthew M. Binns, Bhanu P. Chowdhary, Maren Scharfe, Michael Jarek, Gabriele Nordsiek, Frank Schrader, Helmut Blöcker

Research output: Contribution to journalArticlepeer-review

48 Citations (Scopus)

Abstract

In an effort to increase the density of sequence-based markers for the horse genome we generated 9473 BAC end sequences (BESs) from the CHORI-241 BAC library with an average read length of 677 bp. BLASTN searches with the BESs revealed 4036 meaningful hits (E ≤ 10-5) in the human genome that provide useful markers for the human-horse comparative map. The 4036 BLASTN hits allowed the anchoring of 3079 BAC clones to the human genome, on average one corresponding equine BAC clone per megabase of human DNA. We used the BLASTN anchored BESs for an in silico prediction of the gene content and chromosome assignment of comparatively mapped equine BAC clones. As a first verification of our in silico mapping strategy we placed 19 equine BESs with matches to HSA6 onto the RH map. All markers were assigned to the predicted localizations on ECA10, ECA20, and ECA31, respectively.

Original languageEnglish
Pages (from-to)772-776
Number of pages5
JournalGenomics
Volume87
Issue number6
DOIs
Publication statusPublished - Jun 2006
Externally publishedYes

Keywords

  • BES
  • BLASTN
  • Comparative map
  • Database
  • Horse
  • Physical map
  • RH map

ASJC Scopus subject areas

  • Genetics

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